| Gene | Arrb2 |
| Chromosome | 11 |
| Deletion Start | 70,440,358 |
| Deletion End | 70,432,734 |
| Deletion Size | 7,625 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,432,983 | 70,433,003 | TGGGTTCTCGCAGAGATGTAG |
| TUP | 70,433,005 | 70,433,030 | AGTCGGACTACTGGGTTCTCTGTGCA |
| TUR | 70,433,033 | 70,433,050 | TCCGCCCTTGCATCACAG |
| TDF | 70,440,157 | 70,440,176 | CACCAAGCTATGCCACAGAC |
| TDP | 70,440,185 | 70,440,208 | CGTGTTTGAGGACTTTGCCCGGCT |
| TDR | 70,440,219 | 70,440,243 | CATCACAGTCATCATCCTTCATTCC |
PCR Primers
| Primer | Sequence |
| SU | AATCTAGTTCTAAAAGCTTGAGG |
| SD | GATGGGTACACTGGACACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17027A-A8 | VGB6 | C57BL/6NTac | |
| 17027A-E4 | VGB6 | C57BL/6NTac | |
| 17027A-F1 | VGB6 | C57BL/6NTac | |
| 17027A-F6 | VGB6 | C57BL/6NTac | |
| 17027A-H11 | VGB6 | C57BL/6NTac | |
| 17027A-H12 | VGB6 | C57BL/6NTac | |
