| Gene | Adcyap1 |
| Chromosome | 17 |
| Deletion Start | 93,204,193 |
| Deletion End | 93,200,017 |
| Deletion Size | 4,177 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,200,072 | 93,200,092 | GCAGTGTCTCCTGTTCACCTG |
| TUP | 93,200,097 | 93,200,119 | CGGACTCAGCTTCCCTGGGATCA |
| TUR | 93,200,162 | 93,200,180 | GGTGACCAAAGTGGGTGTC |
| TDF | 93,203,493 | 93,203,513 | GCCCACGTTTGCCTAGATTTG |
| TDP | 93,203,518 | 93,203,547 | ATCAATGATCTGATAGAAGCCCACCGGCCT |
| TDR | 93,203,560 | 93,203,581 | GCCTTTGCACTACCCGACATAC |
PCR Primers
| Primer | Sequence |
| SU | CAACCCTTTCCTAGACACCG |
| SD | TGGTATCTTCAAGACGGTTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17031A-A10 | VGB6 | C57BL/6NTac | |
| 17031A-F11 | VGB6 | C57BL/6NTac | |
