| Gene | Ciita |
| Chromosome | 16 |
| Deletion Start | 10,525,318 |
| Deletion End | 10,488,376 |
| Deletion Size | 36,943 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,488,848 | 10,488,869 | GCACCTGCTTTGAAGACAACAC |
| TUP | 10,488,873 | 10,488,894 | TGGCCTTACCGCCAAGCTACAC |
| TUR | 10,488,896 | 10,488,915 | GCAACACAGATGCAGCCAAG |
| TDF | 10,525,114 | 10,525,135 | CCTCGGTGTCCTGCTATGTTTC |
| TDP | 10,525,137 | 10,525,160 | TCTAACCTGGCCCTGTGGTCCCAT |
| TDR | 10,525,161 | 10,525,182 | AGTGGGTGTCCACATTCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | CAGTGACTGAGCTGAGGTGC |
| SD | CTTCCAGAGAAGCAGCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17035A-C2 | VGB6 | C57BL/6NTac | |
| 17035A-C5 | VGB6 | C57BL/6NTac | |
