| Gene | Tgfb2 |
| Chromosome | 1 |
| Deletion Start | 186,704,771 |
| Deletion End | 186,704,433 |
| Deletion Size | 339 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 186,704,707 | 186,704,726 | GTGGCGCTCAGTCTGTCTAC |
| TUP | 186,704,682 | 186,704,706 | CTGCAGCACCCTCGACATGGATCAG |
| TUR | 186,704,627 | 186,704,648 | GCTTCAGCTTGCTCAGGATCTG |
| TDF | 186,704,557 | 186,704,580 | GGAGGTGATTTCCATCTACAACAG |
| TDP | 186,704,538 | 186,704,556 | TACCAGGGACTTACTGCAG |
| TDR | 186,704,520 | 186,704,537 | CCTCCGGCTTGCCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CTTCAGAGCCACTCACCCTC |
| SD | ATTTGCTAGTTGGTAGACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 61 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17042A-B2 | VGB6 | C57BL/6NTac | |
| 17042A-H9 | VGB6 | C57BL/6NTac | |
| 17042A-B11 | VGB6 | C57BL/6NTac | |
| 17042A-C7 | VGB6 | C57BL/6NTac | |
| 17042A-F5 | VGB6 | C57BL/6NTac | |
| 17042A-D11 | VGB6 | C57BL/6NTac | |
