| Gene | Tgfb3 |
| Chromosome | 12 |
| Deletion Start | 86,058,261 |
| Deletion End | 86,057,917 |
| Deletion Size | 345 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,077,900 | 86,077,919 | TGCAAAGGGCTCTGGTAGTC |
| TUP | 86,077,872 | 86,077,895 | CCCTGCTGAACTTGGCCACAATCA |
| TUR | 86,077,840 | 86,077,858 | GTCCAACGTGGTGCAAGTG |
| TDF | 86,077,714 | 86,077,736 | CCTATCAGGTCCTGGCACTTTAC |
| TDP | 86,077,691 | 86,077,712 | ACAGCACCCGGGAGTTGCTGGA |
| TDR | 86,077,589 | 86,077,608 | GCCAGTCCCTGGATCATGTC |
PCR Primers
| Primer | Sequence |
| SU | ACAAGGATCCTAGCTCTACC |
| SD | TCCATCAAGGATGCCATAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 148 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17043A-C4 | VGB6 | C57BL/6NTac | |
| 17043A-C12 | VGB6 | C57BL/6NTac | |
| 17043A-D3 | VGB6 | C57BL/6NTac | |
| 17043A-H6 | VGB6 | C57BL/6NTac | |
| 17043A-E5 | VGB6 | C57BL/6NTac | |
| 17043A-E8 | VGB6 | C57BL/6NTac | |
