| Gene | Cntfr |
| Chromosome | 4 |
| Deletion Start | 41,675,101 |
| Deletion End | 41,658,140 |
| Deletion Size | 16,963 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,674,845 | 41,674,865 | CAGGCTGACCCACCAATTCTC |
| TUP | 41,674,819 | 41,674,843 | CAGGCACTACCCTCATCTGGGTGGT |
| TUR | 41,674,756 | 41,674,779 | GACCCTCTACCTTCCTTCCCTATG |
| TDF | 41,658,896 | 41,658,918 | ACGCAGGGAAGGAATACATCATC |
| TDP | 41,658,873 | 41,658,895 | CAGGTGGCAGCCAAGGACAATGA |
| TDR | 41,658,852 | 41,658,872 | CAGTCACTCCATGTCCCAATC |
PCR Primers
| Primer | Sequence |
| SU | AAGGCGATCTAGAGACGTGC |
| SD | CAACAAAGACCCTGGGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17044A-B11 | VGB6 | C57BL/6NTac | |
| 17044B-C2 | VGB6 | C57BL/6NTac | |
| 17044B-D8 | VGB6 | C57BL/6NTac | |
