| Gene | S1pr5 |
| Chromosome | 9 |
| Deletion Start | 21,245,125 |
| Deletion End | 21,243,872 |
| Deletion Size | 1,254 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,244,958 | 21,244,977 | TGCTGGAGAACTTGGCTGTG |
| TUP | 21,244,936 | 21,244,956 | TCTTGGTGCTGGTCCGCCACC |
| TUR | 21,244,877 | 21,244,897 | GAGCAGGTCCGACAAAGTGAG |
| TDF | 21,244,365 | 21,244,384 | GCCCTGCTTCGTACCCTTAG |
| TDP | 21,244,336 | 21,244,355 | CCTGGCCTTCGTGGCCTGCT |
| TDR | 21,244,297 | 21,244,321 | ACGCGACATCCAGTAATAGCAAGAG |
PCR Primers
| Primer | Sequence |
| SU | TAGTCTCCGTGTGGAGTGTC |
| SD | TGGAGGTCTGAATCCAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17045A-A9 | VGB6 | C57BL/6NTac | |
| 17045A-D1 | VGB6 | C57BL/6NTac | |
| 17045A-D11 | VGB6 | C57BL/6NTac | |
| 17045A-F11 | VGB6 | C57BL/6NTac | |
| 17045A-D3 | VGB6 | C57BL/6NTac | |
