| Gene | Sostdc1 |
| Chromosome | 12 |
| Deletion Start | 36,317,474 |
| Deletion End | 36,314,297 |
| Deletion Size | 3,178 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,314,621 | 36,314,640 | GGAGCAATCTGGTGCATGTG |
| TUP | 36,314,641 | 36,314,664 | TAGGCTGGCGTTCTTTCCTGGGTA |
| TUR | 36,314,717 | 36,314,737 | GGGCACTGGATAAACCATGTC |
| TDF | 36,317,028 | 36,317,046 | GCAGGTCGAGTTCAAGTGG |
| TDP | 36,317,048 | 36,317,069 | CTGCAGGGAACTGCGGTCCACC |
| TDR | 36,317,093 | 36,317,111 | CAGAGGGCTGATGCTGGTG |
PCR Primers
| Primer | Sequence |
| SU | GGCTGAGGAGCAAGTGGTAG |
| SD | CGAACTTCTCTGGTTCGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17048A-A3 | VGB6 | C57BL/6NTac | |
| 17048A-D7 | VGB6 | C57BL/6NTac | |
| 17048A-F11 | VGB6 | C57BL/6NTac | |
| 17048A-H7 | VGB6 | C57BL/6NTac | |
