| Gene | Efna1 |
| Chromosome | 3 |
| Deletion Start | 89,279,539 |
| Deletion End | 89,272,476 |
| Deletion Size | 7,064 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,279,471 | 89,279,488 | GACCGCCACATCGTCTTC |
| TUP | 89,279,443 | 89,279,470 | TGGAACAGTTCAAATCCCAAGTAAGCCC |
| TUR | 89,279,391 | 89,279,409 | ACCCTGCGAAAGGCCATTG |
| TDF | 89,272,972 | 89,272,993 | TGCTTGAAGCTGAAGGTGACTG |
| TDP | 89,272,934 | 89,272,962 | AAATCAGTGAGTGTCAGGCCCTGTGGACC |
| TDR | 89,272,906 | 89,272,924 | ACCACGCCCAGCACAGATG |
PCR Primers
| Primer | Sequence |
| SU | TTCCTCCACCTCCAACGTAG |
| SD | TGCACATTATGGCTTCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17049A-G9 | VGB6 | C57BL/6NTac | |
| 17049A-G6 | VGB6 | C57BL/6NTac | |
| 17049A-D1 | VGB6 | C57BL/6NTac | |
| 17049A-F12 | VGB6 | C57BL/6NTac | |
| 17049A-A4 | VGB6 | C57BL/6NTac | |
