| Gene | Lgi1 |
| Chromosome | 19 |
| Deletion Start | 38,306,479 |
| Deletion End | 38,264,886 |
| Deletion Size | 41,594 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,264,916 | 38,264,935 | GCCTGCATTCCCCTGAAAAG |
| TUP | 38,264,939 | 38,264,968 | TGCCTATTTCCTATGCCTCTTTTCTGTGGT |
| TUR | 38,264,984 | 38,265,003 | TTGGCTTCGCTGGTTTCTTC |
| TDF | 38,305,518 | 38,305,540 | GGTTTGGCCTCCCTTTCATAATC |
| TDP | 38,305,544 | 38,305,573 | AGGTTGAGAACCATGGGTTTACATGTTCCA |
| TDR | 38,305,574 | 38,305,596 | GTTAGATGTGTAGCCACACTCTG |
PCR Primers
| Primer | Sequence |
| SU | ACATCACTTCGCTTGCCTTC |
| SD | GGCAGTTGTTTCTGAACACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17051A-C12 | VGB6 | C57BL/6NTac | |
