17052   Tctn1     Status: ES cell colonies screened / QC positives

GeneTctn1
Chromosome5
Deletion Start122,264,366
Deletion End122,245,507
Deletion Size18,860
Genome Build38
MGI IDMGI:3603820
NCBI Gene ID654470
BAC ID416A20
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF122,264,317122,264,338TGGTGCTCCTAAACTGCTACAC
TUP122,264,292122,264,315TCTTCGAGCACCCAGGTCATTGCC
TUR122,264,236122,264,258GCCTTGGTGGAATTGAGATCCTC
TDF122,245,862122,245,882CACTGTGTTGCCTGGCTTCTG
TDP122,245,834122,245,861CCCATGCCACAATGTTCATAGAGGGAGA
TDR122,245,785122,245,806TGGTCATGGAGATGGTCCAAAG

PCR Primers

PrimerSequence
SUAGCACCCAAAGTGTCACAAC
SDTAGTCAAAGGCACTGTGAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR103 bp++-
TDFTDR98 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17052A-C3VGB6C57BL/6NTac
17052A-C12VGB6C57BL/6NTac
17052A-E10VGB6C57BL/6NTac
17052A-E2VGB6C57BL/6NTac
17052A-F12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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