| Gene | Igfbp1 |
| Chromosome | 11 |
| Deletion Start | 7,202,071 |
| Deletion End | 7,197,962 |
| Deletion Size | 4,110 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,198,267 | 7,198,286 | GGGAGCCTGTGTACCAGAAC |
| TUP | 7,198,293 | 7,198,313 | CACCCGCCACGAGCACCTTGT |
| TUR | 7,198,360 | 7,198,380 | CACGTATGTCCCAGCCAAGAG |
| TDF | 7,201,759 | 7,201,778 | CCAGAAGGGCATTGGCAGTG |
| TDP | 7,201,784 | 7,201,809 | TGTTAGCCAGTGGGTATCTGTGCAGT |
| TDR | 7,201,847 | 7,201,867 | GGTCAAGAGCAGCAAGAGATG |
PCR Primers
| Primer | Sequence |
| SU | GCAGCTTGCTGACAGAGTTG |
| SD | CAGAACTTTGCAAGAGCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17053B-H11 | VGB6 | C57BL/6NTac | |
| 17053C-B9 | VGB6 | C57BL/6NTac | |
