| Gene | Agr2 |
| Chromosome | 12 |
| Deletion Start | 36,003,918 |
| Deletion End | 35,995,520 |
| Deletion Size | 8,399 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,995,530 | 35,995,550 | CAGTGTCTGCAATCCTGCTTC |
| TUP | 35,995,552 | 35,995,576 | TGTGGCCATTTCTGGTACCTTGGCC |
| TUR | 35,995,580 | 35,995,600 | TCCAGATTTGACTGTGGTGTC |
| TDF | 36,003,209 | 36,003,231 | TGCTTCTGCACTAGCCACAATAG |
| TDP | 36,003,234 | 36,003,260 | TGATGCTGACAGAGCTTGAATGGGTTC |
| TDR | 36,003,296 | 36,003,314 | GCCCGGTTCTCAGTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CCCACATTACCTTCCAGCAC |
| SD | AACAAACCAGAACCAAATAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17054A-D1 | VGB6 | C57BL/6NTac | |
| 17054A-H8 | VGB6 | C57BL/6NTac | |
| 17054A-G6 | VGB6 | C57BL/6NTac | |
