| Gene | Txndc5 |
| Chromosome | 13 |
| Deletion Start | 38,528,405 |
| Deletion End | 38,501,556 |
| Deletion Size | 26,850 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,527,634 | 38,527,656 | GCCTTTAACTCTTTCACCTCCTG |
| TUP | 38,527,592 | 38,527,619 | CCGGTCATTTGGAAAGTGTGAGGTCGAA |
| TUR | 38,527,552 | 38,527,576 | AAGAATGGAAGCCTGAAGTTATGAC |
| TDF | 38,501,620 | 38,501,642 | AGAGACCTCGACTCCTTACACAG |
| TDP | 38,501,596 | 38,501,618 | TTTGTTCTGCGCCAGGCAAAGGA |
| TDR | 38,501,556 | 38,501,574 | GCCCAGGCTGGATGACTTC |
PCR Primers
| Primer | Sequence |
| SU | AGGTGCTGCACAAGGACC |
| SD | GTTCAGCAATTTGGCTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17055C-A7 | VGB6 | C57BL/6NTac | |
| 17055C-A8 | VGB6 | C57BL/6NTac | |
| 17055C-D9 | VGB6 | C57BL/6NTac | |
| 17055C-H8 | VGB6 | C57BL/6NTac | |
