| Gene | Ins2 |
| Chromosome | 7 |
| Deletion Start | 142,679,530 |
| Deletion End | 142,678,625 |
| Deletion Size | 906 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,679,510 | 142,679,528 | CCTGTGGATGCGCTTCCTG |
| TUP | 142,679,484 | 142,679,506 | CTGCTGGCCCTGCTCTTCCTCTG |
| TUR | 142,679,437 | 142,679,458 | AACCACAAAGGTGCTGCTTGAC |
| TDF | 142,678,762 | 142,678,785 | GAAGCGTGGCATTGTAGATCAGTG |
| TDP | 142,678,740 | 142,678,760 | TGCACCAGCATCTGCTCCCTC |
| TDR | 142,678,698 | 142,678,719 | TGGGTAGTGGTGGGTCTAGTTG |
PCR Primers
| Primer | Sequence |
| SU | CAGACCTAGCACCAGGGAAG |
| SD | ATCAGCTCCTAGCTGCCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17057A-A1 | VGB6 | C57BL/6NTac | |
| 17057A-B9 | VGB6 | C57BL/6NTac | |
| 17057A-E1 | VGB6 | C57BL/6NTac | |
| 17057A-G5 | VGB6 | C57BL/6NTac | |
| 17057A-G8 | VGB6 | C57BL/6NTac | |
| 17057A-F2 | VGB6 | C57BL/6NTac | |
