17059   Tnfrsf1a     Status: ES cell colonies screened / QC positives

GeneTnfrsf1a
Chromosome6
Deletion Start125,361,994
Deletion End125,350,027
Deletion Size11,968
Genome Build38
MGI IDMGI:1314884
NCBI Gene ID21937
BAC ID60B18
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,350,448125,350,465TGGGAGTGGACCCGAAAC
TUP125,350,469125,350,491ACAAAGCTGGGCAGCCTTCCGGT
TUR125,350,522125,350,542CAGAAATGGAGGCGGCAGAAG
TDF125,361,100125,361,120GATCCCGTGCCTGTCAAAGAG
TDP125,361,122125,361,145TGAGAAGGGACGACTCCAGCTTCC
TDR125,361,146125,361,166GGCGTTGGAAGGAGTAGTCAG

PCR Primers

PrimerSequence
SUGAACTCTGGCTCATGATCG
SDACTGAGGAGGCCCTGAGAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR95 bp++-
TDFTDR67 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17059A-B1VGB6C57BL/6NTac
17059A-G1VGB6C57BL/6NTac
17059A-F1VGB6C57BL/6NTac
17059A-C12VGB6C57BL/6NTac
17059A-E2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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