| Gene | St14 |
| Chromosome | 9 |
| Deletion Start | 31,131,623 |
| Deletion End | 31,089,823 |
| Deletion Size | 41,801 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,131,041 | 31,131,061 | AAGGTGGCTGTCTGGGTAGTG |
| TUP | 31,131,014 | 31,131,039 | TGAAGCATGTGTCCTGAGCCTTCTGG |
| TUR | 31,130,983 | 31,131,001 | GCTTCTGCTTCCCGCTTAC |
| TDF | 31,090,159 | 31,090,179 | GAAGTCATGCTTCCGGGAGAG |
| TDP | 31,090,132 | 31,090,157 | TCAGTTGGCCATGGGAGAGGCTACTT |
| TDR | 31,090,111 | 31,090,130 | TCTTAGTAGCTGCCTCAACC |
PCR Primers
| Primer | Sequence |
| SU | TGTGCTCCAGGAGAAAGAGC |
| SD | GGACAGCATCATCTTCACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17063A-G5 | VGB6 | C57BL/6NTac | |
| 17063B-E4 | VGB6 | C57BL/6NTac | |
