| Gene | Timm50 |
| Chromosome | 7 |
| Deletion Start | 28,312,029 |
| Deletion End | 28,305,853 |
| Deletion Size | 6,177 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,311,563 | 28,311,583 | CTCCTTGAGCGAGAGGAACAG |
| TUP | 28,311,540 | 28,311,561 | AAGGATCCAGCCTGCCGTCCGT |
| TUR | 28,311,506 | 28,311,528 | TCATGGACTTGGAGGTCAGAATG |
| TDF | 28,306,436 | 28,306,455 | GCATTCAAACAGCGGCAGAG |
| TDP | 28,306,412 | 28,306,435 | CCGGCTTGAGCAGGTTAGGCTCTA |
| TDR | 28,306,361 | 28,306,380 | GAGGCCAAAGCCAAGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | TAACGATGGGAGGTGAAAGG |
| SD | CTCCAGAACCACATTCCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17066A-A2 | VGB6 | C57BL/6NTac | |
| 17066A-B7 | VGB6 | C57BL/6NTac | |
| 17066A-D12 | VGB6 | C57BL/6NTac | |
| 17066A-H4 | VGB6 | C57BL/6NTac | |
| 17066A-H10 | VGB6 | C57BL/6NTac | |
