| Gene | Dll3 |
| Chromosome | 7 |
| Deletion Start | 28,301,743 |
| Deletion End | 28,293,899 |
| Deletion Size | 7,845 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,300,864 | 28,300,886 | TGGCTGTCTCTGAGTTCACTATG |
| TUP | 28,300,834 | 28,300,862 | AGCTAAATCTGGTCGTGAGTTCCTGATCC |
| TUR | 28,300,794 | 28,300,814 | AACTCCAGCACTTGGGAGATG |
| TDF | 28,294,478 | 28,294,496 | CGCCGCACTCTTGGTCATC |
| TDP | 28,294,457 | 28,294,476 | ACGTTCGCCGCCGAGGTCCT |
| TDR | 28,294,356 | 28,294,376 | CCAGCACCGTCTTGTAACCTC |
PCR Primers
| Primer | Sequence |
| SU | ATGGGTTCATAGGGTCCTCC |
| SD | TCCAAGCTATCACAAGATGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 141 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17067A-A11 | VGB6 | C57BL/6NTac | |
| 17067A-C7 | VGB6 | C57BL/6NTac | |
| 17067A-D3 | VGB6 | C57BL/6NTac | |
| 17067A-E4 | VGB6 | C57BL/6NTac | |
| 17067A-G10 | VGB6 | C57BL/6NTac | |
| 17067A-F1 | VGB6 | C57BL/6NTac | |
