| Gene | Muc1 |
| Chromosome | 3 |
| Deletion Start | 89,233,191 |
| Deletion End | 89,229,123 |
| Deletion Size | 4,069 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,229,175 | 89,229,194 | AAGGTGAGAGGCGCAAGGTG |
| TUP | 89,229,203 | 89,229,225 | CTGCGCTGTTCAGGTGGGACTCC |
| TUR | 89,229,230 | 89,229,253 | CCAGTAAGCAAACTCCACAGAAAG |
| TDF | 89,232,336 | 89,232,357 | GCCGTCTACCTGTACCATTCTG |
| TDP | 89,232,360 | 89,232,385 | CTCATTAGGATGCCCAGAGGTGAGGC |
| TDR | 89,232,417 | 89,232,435 | GTCAGCGGGAAATGCAGTG |
PCR Primers
| Primer | Sequence |
| SU | ATGGACTTCCCATGTCTTGC |
| SD | CAGTTCTTTCTCAGGCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17068A-A6 | VGB6 | C57BL/6NTac | |
| 17068A-A12 | VGB6 | C57BL/6NTac | |
| 17068A-B9 | VGB6 | C57BL/6NTac | |
| 17068A-A8 | VGB6 | C57BL/6NTac | |
