| Gene | Nodal |
| Chromosome | 10 |
| Deletion Start | 61,424,713 |
| Deletion End | 61,418,303 |
| Deletion Size | 6,411 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,419,123 | 61,419,142 | ACCCAGCGACCTGAGTGATG |
| TUP | 61,419,143 | 61,419,167 | CTCACCAGAGTAGGCCGACAGGTGG |
| TUR | 61,419,213 | 61,419,235 | CCCAAGAGGCGAGATGTTGATTG |
| TDF | 61,423,961 | 61,423,982 | GCTGATCCTCTTGCCTTCACAC |
| TDP | 61,423,985 | 61,424,008 | CTCGTCTGGGATCACAGGCATGCA |
| TDR | 61,424,028 | 61,424,046 | GGGCGAGGTCCTGGATTTG |
PCR Primers
| Primer | Sequence |
| SU | AGACTTCCCGAGGTAGAGGG |
| SD | TTGTGGGTCTTCCCTACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17069A-C12 | VGB6 | C57BL/6NTac | |
| 17069A-F5 | VGB6 | C57BL/6NTac | |
| 17069A-H8 | VGB6 | C57BL/6NTac | |
| 17069A-G5 | VGB6 | C57BL/6NTac | |
| 17069A-B4 | VGB6 | C57BL/6NTac | |
