| Gene | Shh |
| Chromosome | 5 |
| Deletion Start | 28,466,760 |
| Deletion End | 28,457,816 |
| Deletion Size | 8,945 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,466,597 | 28,466,616 | CCCAACGTAGCCGAGAAGAC |
| TUP | 28,466,560 | 28,466,587 | CAGCGGCAGATATGAAGGGAAGATCACA |
| TUR | 28,466,535 | 28,466,558 | GTTCCTTAAATCGTTCGGAGTTTC |
| TDF | 28,457,937 | 28,457,955 | CGGGCATCCACTGGTACTC |
| TDP | 28,457,910 | 28,457,935 | CAGCTGCTCTACCACATTGGCACCTG |
| TDR | 28,457,857 | 28,457,877 | AGCTGGACTTGACCGCCATTC |
PCR Primers
| Primer | Sequence |
| SU | GCCTGTCTCACAAGCTCTCC |
| SD | GCATAGCAGGAGAGGAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17071A-A6 | VGB6 | C57BL/6NTac | |
| 17071A-E4 | VGB6 | C57BL/6NTac | |
