| Gene | Tspan8 |
| Chromosome | 10 |
| Deletion Start | 115,849,488 |
| Deletion End | 115,817,604 |
| Deletion Size | 31,885 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,818,004 | 115,818,024 | ATGATGCCAATCAGCACTCTG |
| TUP | 115,818,034 | 115,818,063 | TCCAAAGAAAACAGTTTGATTCAGCATGGC |
| TUR | 115,818,066 | 115,818,087 | GATGTCAAAGGGAGGGAACCAG |
| TDF | 115,849,057 | 115,849,080 | TCCCAGACCATCCCAGAGTAATAG |
| TDP | 115,849,082 | 115,849,111 | TGGTACTGCACAATTAAGTAGGGCCATTGA |
| TDR | 115,849,113 | 115,849,130 | CCAGCTTGCCAGCCAATC |
PCR Primers
| Primer | Sequence |
| SU | TCCGCAGGGTTAGAAGAGAG |
| SD | TCAGCTGTTGACATACCTGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17072A-E5 | VGB6 | C57BL/6NTac | |
| 17072A-G4 | VGB6 | C57BL/6NTac | |
| 17072A-F5 | VGB6 | C57BL/6NTac | |
