| Gene | Mesdc2 |
| Chromosome | 7 |
| Deletion Start | 83,898,069 |
| Deletion End | 83,892,293 |
| Deletion Size | 5,777 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,892,418 | 83,892,439 | GAAGGACATCCGCGATTACAAC |
| TUP | 83,892,442 | 83,892,462 | TGCCGACATGGCGCGACTTCT |
| TUR | 83,892,492 | 83,892,513 | GTATGGGAGATGCTCAGAAGTG |
| TDF | 83,897,637 | 83,897,654 | TGGCCCAAGGCAGAACAG |
| TDP | 83,897,663 | 83,897,690 | TTGGGTGGAATTGAAATGTAGGCCTCCC |
| TDR | 83,897,726 | 83,897,746 | GCCAGAGAAGCCAGCAGTTAG |
PCR Primers
| Primer | Sequence |
| SU | AAGTGGTGAAAGGCTCGATG |
| SD | GATACAACAGGCCATGGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17073C-E9 | VGB6 | C57BL/6NTac | |
| 17073C-D4 | VGB6 | C57BL/6NTac | |
