| Gene | Tspan4 |
| Chromosome | 7 |
| Deletion Start | 141,493,000 |
| Deletion End | 141,482,507 |
| Deletion Size | 10,494 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,482,957 | 141,482,976 | ATGGACATGGGTGGCACCTG |
| TUP | 141,482,979 | 141,482,999 | TCCCTGCCACCTCAGCAGAGC |
| TUR | 141,483,042 | 141,483,064 | GGCCCATCTTCTGCTCTATCATC |
| TDF | 141,492,856 | 141,492,875 | ACTCTGCCTGTGACCTCTTG |
| TDP | 141,492,876 | 141,492,899 | TCTCCACAGATTCTGGGCCTCACC |
| TDR | 141,492,921 | 141,492,939 | TGTCCGCCTTTACCACCTG |
PCR Primers
| Primer | Sequence |
| SU | ACGGACGAATCCACGTAC |
| SD | CATCTGGAACCACGACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17076A-D3 | VGB6 | C57BL/6NTac | |
| 17076A-G5 | VGB6 | C57BL/6NTac | |
| 17076A-F8 | VGB6 | C57BL/6NTac | |
| 17076A-H12 | VGB6 | C57BL/6NTac | |
