| Gene | March3 |
| Chromosome | 18 |
| Deletion Start | 56,812,016 |
| Deletion End | 56,783,024 |
| Deletion Size | 28,993 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,811,984 | 56,812,002 | CAGTCACCTGCCCGAAGTC |
| TUP | 56,811,963 | 56,811,983 | TTGCCAGACTGCACCAGCTCA |
| TUR | 56,811,897 | 56,811,918 | GACATACTGTGGCTGCCCATTC |
| TDF | 56,783,724 | 56,783,745 | AGGGTCAGAGCATGTGTCAAAG |
| TDP | 56,783,693 | 56,783,719 | TGGTATCATCCTAAGGAGGCAAGGGAC |
| TDR | 56,783,649 | 56,783,669 | ACCAGAATCAGGGCCTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GCAGAGCTCCTGACTGTGTG |
| SD | CTTACACCAGCTGGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17077A-A7 | VGB6 | C57BL/6NTac | |
| 17077A-B4 | VGB6 | C57BL/6NTac | |
| 17077A-F12 | VGB6 | C57BL/6NTac | |
| 17077A-G4 | VGB6 | C57BL/6NTac | |
| 17077A-H6 | VGB6 | C57BL/6NTac | |
| 17077A-E7 | VGB6 | C57BL/6NTac | |
