| Gene | March5 |
| Chromosome | 19 |
| Deletion Start | 37,221,524 |
| Deletion End | 37,207,873 |
| Deletion Size | 13,652 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,208,460 | 37,208,484 | GGTCACACTAGAGTAGAGGTTATGG |
| TUP | 37,208,488 | 37,208,516 | ACAGCCACAAGCATGCAACATTGAACACG |
| TUR | 37,208,544 | 37,208,562 | ACAAGCCCAAACGTGTCTG |
| TDF | 37,221,170 | 37,221,193 | GGGAAGAAAGCTACACAAGTGATG |
| TDP | 37,221,198 | 37,221,225 | AACAGGGCCTCTTCCTTTGCTACTTGAA |
| TDR | 37,221,231 | 37,221,252 | GAGGGCAGAAGTTGGGAAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CGGTCCGAGGACTACATCTC |
| SD | GCCCTCACAGTTCCTTCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17078A-G3 | VGB6 | C57BL/6NTac | |
| 17078A-H12 | VGB6 | C57BL/6NTac | |
| 17078A-B8 | VGB6 | C57BL/6NTac | |
| 17078A-H11 | VGB6 | C57BL/6NTac | |
| 17078A-E6 | VGB6 | C57BL/6NTac | |
| 17078A-G9 | VGB6 | C57BL/6NTac | |
