17080   Tmem198b     Status: ES cell colonies screened / QC positives

GeneTmem198b
Chromosome10
Deletion Start128,803,269
Deletion End128,801,060
Deletion Size2,210
Genome Build38
MGI IDMGI:1921077
NCBI Gene ID73827
BAC ID359H12
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF128,802,638128,802,661TCACTGGGCTTACACTTCTCTTTC
TUP128,802,614128,802,637CCCTTTCCTTGAGGTTGCCACGGT
TUR128,802,564128,802,587CGTATTGGGAGAGGAACAGCTAAC
TDF128,801,132128,801,152GGATTCTGATTGCAGCTCCAC
TDP128,801,110128,801,131TGGCCTGCTTACCACACCTCAC
TDR128,801,084128,801,103GGAGGTTAGGCTCAGGTCTG

PCR Primers

PrimerSequence
SUGTGCCTAGGACCTCTGGGAG
SDGGGAATGGGTTGTAGTTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR98 bp++-
TDFTDR69 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17080A-B12VGB6C57BL/6NTac
17080A-C5VGB6C57BL/6NTac
17080A-H4VGB6C57BL/6NTac
17080A-D5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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