| Gene | 2010107E04Rik |
| Chromosome | 12 |
| Deletion Start | 111,963,350 |
| Deletion End | 111,961,456 |
| Deletion Size | 1,895 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,962,959 | 111,962,976 | TACCTGCTGAGCCACCTG |
| TUP | 111,962,929 | 111,962,957 | CTGGTCCATAACGTGGCCTTAGATGTTCC |
| TUR | 111,962,868 | 111,962,888 | GCTCTCCCAACAAGGGCTATG |
| TDF | 111,961,543 | 111,961,566 | CTGTACCACTTGTTCCTCTCCTTG |
| TDP | 111,961,519 | 111,961,541 | AAGGTCCTGCACCTGCCCATGGC |
| TDR | 111,961,493 | 111,961,513 | TTGTGGATTCGGAGCAGTTAG |
PCR Primers
| Primer | Sequence |
| SU | ACATCTGACCGTGGTGTGTG |
| SD | GGCAGAAGCAGAACCACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17082A-A2 | VGB6 | C57BL/6NTac | |
| 17082A-A4 | VGB6 | C57BL/6NTac | |
| 17082A-A6 | VGB6 | C57BL/6NTac | |
| 17082A-B10 | VGB6 | C57BL/6NTac | |
| 17082A-F12 | VGB6 | C57BL/6NTac | |
