| Gene | 2310003L22Rik |
| Chromosome | 2 |
| Deletion Start | 140,203,702 |
| Deletion End | 140,188,760 |
| Deletion Size | 14,943 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,189,600 | 140,189,617 | CAGGCCCATGTGGAAGTC |
| TUP | 140,189,620 | 140,189,645 | TGTGACTTTGAAGTGCTGCTTCTGCC |
| TUR | 140,189,701 | 140,189,720 | AGGCAGGGCAAGGATTCAAG |
| TDF | 140,202,869 | 140,202,890 | TGGGTGAGAGCAACTGTTCTTG |
| TDP | 140,202,900 | 140,202,922 | AGCCCTGCTGCACCGAGACACAA |
| TDR | 140,202,981 | 140,203,001 | CACGCAGCATAAACCCATCTG |
PCR Primers
| Primer | Sequence |
| SU | GGTGACCTATTACTCTTAAC |
| SD | GCACAGGAACTCTCAGGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 133 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17083A-A11 | VGB6 | C57BL/6NTac | |
| 17083A-B11 | VGB6 | C57BL/6NTac | |
| 17083A-E1 | VGB6 | C57BL/6NTac | |
| 17083A-F4 | VGB6 | C57BL/6NTac | |
| 17083A-D8 | VGB6 | C57BL/6NTac | |
