| Gene | 4932438H23Rik |
| Chromosome | 16 |
| Deletion Start | 91,056,243 |
| Deletion End | 91,055,452 |
| Deletion Size | 792 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,055,990 | 91,056,011 | GCTGTAAGTCTATCCTGCCTTC |
| TUP | 91,055,963 | 91,055,989 | TGCCATGGAGCAAACCAGCTATCATGG |
| TUR | 91,055,941 | 91,055,962 | TGTGAACCAGATGGTCAGATGG |
| TDF | 91,055,754 | 91,055,774 | TCATCCACAGCAGAAGGGAAG |
| TDP | 91,055,715 | 91,055,744 | TGTACAAAGGCTGGCAAACATGTATGTTCA |
| TDR | 91,055,634 | 91,055,655 | TGGCGAGGCTAGAAATGTTGTC |
PCR Primers
| Primer | Sequence |
| SU | TTAGTCAGCGTGCGTAGGTG |
| SD | GCATTCTGAAAGAAGGTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 141 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17087A-D3 | VGB6 | C57BL/6NTac | |
| 17087A-F7 | VGB6 | C57BL/6NTac | |
