Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

17088 4933434E20Rik Status: ES cell colonies screened / QC positives

Gene	4933434E20Rik
Chromosome	3
Deletion Start	90,061,930
Deletion End	90,053,057
Deletion Size	8,874
Genome Build	38

MGI ID	MGI:1914027
NCBI Gene ID	99650
BAC ID	218M4
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	90,053,213	90,053,237	CTTTGTTCTTCAGCCACTTAACTTG
TUP	90,053,244	90,053,273	TTCTGGACTTCCGAATTGTATCCGTAGACA
TUR	90,053,291	90,053,310	CTTAGAGCCAGGCAGCTTTG
TDF	90,061,637	90,061,658	GGGTCAAAGCACGAATTGGAAG
TDP	90,061,662	90,061,686	TCAGAGACAACACCAGTCAGCAGCC
TDR	90,061,690	90,061,713	CATTTCAGGTGACTGAGTTAGGTC

PCR Primers

Primer	Sequence
SU	GCCACGAAACACTGCTCTTG
SD	TAAGGACTGGGTGAAGGTGG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	98 bp	+	+	-
TDF	TDR	77 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
17088A-G10	VGB6	C57BL/6NTac
17088A-B4	VGB6	C57BL/6NTac
17088A-C3	VGB6	C57BL/6NTac
17088A-C4	VGB6	C57BL/6NTac
17088A-G4	VGB6	C57BL/6NTac