| Gene | Abca8b |
| Chromosome | 11 |
| Deletion Start | 109,981,770 |
| Deletion End | 109,952,557 |
| Deletion Size | 29,214 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,981,374 | 109,981,393 | GAACTGGTTGCCAAGGTGAC |
| TUP | 109,981,345 | 109,981,370 | AGGTGAGAGCAGCACTGTAGACAACA |
| TUR | 109,981,292 | 109,981,310 | TGACTGCCTGTGCTGAGAC |
| TDF | 109,952,649 | 109,952,669 | TGCAACACCAAGAGACTGAAC |
| TDP | 109,952,622 | 109,952,648 | TGCTTCCCAGTTCTGATGGACATCCTG |
| TDR | 109,952,575 | 109,952,592 | GTTTGGATGCGGGCTGAC |
PCR Primers
| Primer | Sequence |
| SU | ACAGCTTAGGAGACCTTGCC |
| SD | CCTTCGTATTAATCACAGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17090A-A12 | VGB6 | C57BL/6NTac | |
| 17090A-F5 | VGB6 | C57BL/6NTac | |
| 17090A-H9 | VGB6 | C57BL/6NTac | |
| 17090A-B11 | VGB6 | C57BL/6NTac | |
| 17090A-C1 | VGB6 | C57BL/6NTac | |
