| Gene | Abcd4 |
| Chromosome | 12 |
| Deletion Start | 84,617,317 |
| Deletion End | 84,603,086 |
| Deletion Size | 14,232 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,617,262 | 84,617,280 | GAGCAGGGCTAGTGAGGAC |
| TUP | 84,617,233 | 84,617,254 | TTGTCAGGGACGCACTGCCTGG |
| TUR | 84,617,131 | 84,617,149 | GGCAGCGTCAACTCCATTC |
| TDF | 84,603,823 | 84,603,840 | ATCCCTCCCTGTGTCCTC |
| TDP | 84,603,799 | 84,603,822 | TACCCGTGATGAGCAGAGCTTGGC |
| TDR | 84,603,731 | 84,603,750 | ACGATCACAAGCCCTCTAAC |
PCR Primers
| Primer | Sequence |
| SU | ACTCGGAACTACCAGTTGGC |
| SD | ATCACCAGTTCTTGAGTCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 150 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17092A-C4 | VGB6 | C57BL/6NTac | |
| 17092A-D5 | VGB6 | C57BL/6NTac | |
| 17092A-G4 | VGB6 | C57BL/6NTac | |
| 17092A-D11 | VGB6 | C57BL/6NTac | |
| 17092A-H9 | VGB6 | C57BL/6NTac | |
