| Gene | Adamts13 |
| Chromosome | 2 |
| Deletion Start | 27,009,530 |
| Deletion End | 26,973,540 |
| Deletion Size | 35,991 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,973,976 | 26,973,996 | CCACCATGCTGCTCCATTCAC |
| TUP | 26,973,997 | 26,974,025 | AGGTAGCTGTCCTGATGTACTCTGAGCCA |
| TUR | 26,974,046 | 26,974,065 | AAGTCCCTCGGTCCCTCATC |
| TDF | 27,009,327 | 27,009,345 | CAGCGAGGCTGAACTGGAG |
| TDP | 27,009,346 | 27,009,368 | TTTAGCCCTGGCTTCCTGGAGGC |
| TDR | 27,009,411 | 27,009,430 | TGTCGTCCTGTTCCGATGTC |
PCR Primers
| Primer | Sequence |
| SU | ACTATATGCGAGCCCACACC |
| SD | TGGGAGAGGTGGTGATTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17094C-B11 | VGB6 | C57BL/6NTac | |
| 17094C-E3 | VGB6 | C57BL/6NTac | |
