| Gene | Cd160 |
| Chromosome | 3 |
| Deletion Start | 96,808,869 |
| Deletion End | 96,800,560 |
| Deletion Size | 8,310 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,808,480 | 96,808,503 | GAGTTTCGTTGGGAGAGTTTATGG |
| TUP | 96,808,455 | 96,808,479 | TGTCCCTCTGCTGGAGAAAGAAGCA |
| TUR | 96,808,431 | 96,808,453 | CCTGCCTCCTGCTTAGTTCTTTG |
| TDF | 96,801,249 | 96,801,268 | CAGCCTTGCACTATCACCAC |
| TDP | 96,801,219 | 96,801,247 | ACCTTTGACTCCAAACCCATCCTAGAACA |
| TDR | 96,801,195 | 96,801,217 | GGGAACCATGAGCAGAGACTTTG |
PCR Primers
| Primer | Sequence |
| SU | TATTCACCAAAGGAGCCCAG |
| SD | AAACATACCTGGCATTCAAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17100A-A7 | VGB6 | C57BL/6NTac | |
| 17100A-B11 | VGB6 | C57BL/6NTac | |
| 17100A-D8 | VGB6 | C57BL/6NTac | |
| 17100A-G12 | VGB6 | C57BL/6NTac | |
| 17100A-G4 | VGB6 | C57BL/6NTac | |
| 17100A-E12 | VGB6 | C57BL/6NTac | |
