| Gene | Cd37 |
| Chromosome | 7 |
| Deletion Start | 45,238,725 |
| Deletion End | 45,233,853 |
| Deletion Size | 4,873 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,238,223 | 45,238,243 | CCAGGCCATCCTAGACTACAC |
| TUP | 45,238,189 | 45,238,212 | AGCCCAGGCTGGATAATGAGGTCC |
| TUR | 45,238,143 | 45,238,162 | CTGTTCCTAACCGCGACCTC |
| TDF | 45,234,723 | 45,234,744 | GTGGCTGCACAACAATATCATC |
| TDP | 45,234,695 | 45,234,722 | TCCATAGTGGGAATCTGTCTGGGAGTCG |
| TDR | 45,234,672 | 45,234,694 | GGGTCAAGTCACCTCAAGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | CGAGGCTAATGGGTGCTAAG |
| SD | TAAGTGAGCCTTGGACCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17101A-A8 | VGB6 | C57BL/6NTac | |
| 17101A-B4 | VGB6 | C57BL/6NTac | |
| 17101A-C3 | VGB6 | C57BL/6NTac | |
| 17101A-C12 | VGB6 | C57BL/6NTac | |
