| Gene | Cercam |
| Chromosome | 2 |
| Deletion Start | 29,881,948 |
| Deletion End | 29,869,541 |
| Deletion Size | 12,408 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,869,816 | 29,869,836 | CACAACCCGAACAAGCTCCTC |
| TUP | 29,869,837 | 29,869,861 | CCTCCCAGACAGCCCTTCAACAGTA |
| TUR | 29,869,904 | 29,869,922 | GAGTGTCCCTGGGCTGAAC |
| TDF | 29,881,349 | 29,881,367 | TTGCATGTGAGTGCAGGTG |
| TDP | 29,881,368 | 29,881,390 | TCCAAAGAGGCCAGAAGAGGGCA |
| TDR | 29,881,400 | 29,881,418 | TCGGGACTCCAGCTCTAAG |
PCR Primers
| Primer | Sequence |
| SU | ATCCCGGGTGTCAGTATTCC |
| SD | CTCTGAGACCTTCAGGAGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17106A-A5 | VGB6 | C57BL/6NTac | |
| 17106A-B12 | VGB6 | C57BL/6NTac | |
| 17106A-E10 | VGB6 | C57BL/6NTac | |
