| Gene | Chrna4 |
| Chromosome | 2 |
| Deletion Start | 181,039,070 |
| Deletion End | 181,024,665 |
| Deletion Size | 14,406 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,038,720 | 181,038,740 | GCATTTCGGAGCTGGAATCAG |
| TUP | 181,038,688 | 181,038,716 | CTCCAAGTAGGATAGAGGTGGAGTCCACC |
| TUR | 181,038,653 | 181,038,673 | AAGGCGAGATTTGGCTGGATG |
| TDF | 181,024,868 | 181,024,886 | TTTGCAGGTGAAGGAGGAC |
| TDP | 181,024,838 | 181,024,864 | AAATACGTGGCCATGGTCATTGACCGA |
| TDR | 181,024,800 | 181,024,823 | CCCAGAAGGCAGACAATGATGAAC |
PCR Primers
| Primer | Sequence |
| SU | CCGAGATGACTGGAATGACC |
| SD | TTTGAGTCAGAGAGCAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17108A-A3 | VGB6 | C57BL/6NTac | |
| 17108A-E6 | VGB6 | C57BL/6NTac | |
| 17108A-E11 | VGB6 | C57BL/6NTac | |
| 17108A-E10 | VGB6 | C57BL/6NTac | |
| 17108A-G2 | VGB6 | C57BL/6NTac | |
