Gene | Chrna4 |
Chromosome | 2 |
Deletion Start | 181,039,070 |
Deletion End | 181,024,665 |
Deletion Size | 14,406 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 181,038,720 | 181,038,740 | GCATTTCGGAGCTGGAATCAG |
TUP | 181,038,688 | 181,038,716 | CTCCAAGTAGGATAGAGGTGGAGTCCACC |
TUR | 181,038,653 | 181,038,673 | AAGGCGAGATTTGGCTGGATG |
TDF | 181,024,868 | 181,024,886 | TTTGCAGGTGAAGGAGGAC |
TDP | 181,024,838 | 181,024,864 | AAATACGTGGCCATGGTCATTGACCGA |
TDR | 181,024,800 | 181,024,823 | CCCAGAAGGCAGACAATGATGAAC |
PCR Primers
Primer | Sequence |
SU | CCGAGATGACTGGAATGACC |
SD | TTTGAGTCAGAGAGCAGCCC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 88 bp | + | + | - |
TDF | TDR | 87 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
17108A-A3 | VGB6 | C57BL/6NTac | |
17108A-E6 | VGB6 | C57BL/6NTac | |
17108A-E11 | VGB6 | C57BL/6NTac | |
17108A-E10 | VGB6 | C57BL/6NTac | |
17108A-G2 | VGB6 | C57BL/6NTac | |