| Gene | Chrnb1 |
| Chromosome | 11 |
| Deletion Start | 69,795,876 |
| Deletion End | 69,784,604 |
| Deletion Size | 11,273 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,795,236 | 69,795,256 | CTGAGCGGAGATTCAGAGTTG |
| TUP | 69,795,213 | 69,795,235 | TATCCATTAGACGCCGGGCGTGG |
| TUR | 69,795,165 | 69,795,182 | GCCTTGGTGCCTGCTTTC |
| TDF | 69,785,065 | 69,785,084 | CCCGACAGCTTCAGGAACAG |
| TDP | 69,785,037 | 69,785,063 | AGGACCACGACGCAGTAAGTCCAAAGA |
| TDR | 69,785,009 | 69,785,027 | GAGGTCCATGAGCCCACAG |
PCR Primers
| Primer | Sequence |
| SU | CTGGCTTGTTGCAAGAGATG |
| SD | ACCGCAGAAGAACAAAACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17109A-B4 | VGB6 | C57BL/6NTac | |
| 17109A-E6 | VGB6 | C57BL/6NTac | |
| 17109A-H7 | VGB6 | C57BL/6NTac | |
