| Gene | Chst5 |
| Chromosome | 8 |
| Deletion Start | 111,890,983 |
| Deletion End | 111,889,747 |
| Deletion Size | 1,237 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,890,798 | 111,890,817 | GCCAGCTCTTCAGCCAACAC |
| TUP | 111,890,771 | 111,890,797 | CCCGATGTCTTCTACCTGATGGAGCCG |
| TUR | 111,890,735 | 111,890,755 | ACTGCCCTGCGACAACGTATC |
| TDF | 111,890,159 | 111,890,177 | GGTGCGCTACGAGGATCTG |
| TDP | 111,890,133 | 111,890,155 | CGGGACCCACTCACCGTAATCCG |
| TDR | 111,890,111 | 111,890,131 | GCCGGTGAAGGCATAGAGTTC |
PCR Primers
| Primer | Sequence |
| SU | CCTTCGCCTAAGCTTCATTG |
| SD | GAGATACTAGGGCGTGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17111C-C4 | VGB6 | C57BL/6NTac | |
| 17111C-F7 | VGB6 | C57BL/6NTac | |
| 17111C-G12 | VGB6 | C57BL/6NTac | |
| 17111C-H11 | VGB6 | C57BL/6NTac | |
| 17111C-H12 | VGB6 | C57BL/6NTac | |
