| Gene | Cx3cl1 |
| Chromosome | 8 |
| Deletion Start | 94,780,569 |
| Deletion End | 94,772,268 |
| Deletion Size | 8,302 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,772,990 | 94,773,011 | CCATTCCACCTCCTTTAACCTC |
| TUP | 94,773,012 | 94,773,035 | TAGAGCTCCATCCTCAGGGAGGGC |
| TUR | 94,773,066 | 94,773,086 | CCACCTTCTCTGAGCGACAAC |
| TDF | 94,779,671 | 94,779,692 | CAAGTTTGAGAAGCGGGTGGAC |
| TDP | 94,779,697 | 94,779,719 | TGACACCTGGGATCACCTTGGCC |
| TDR | 94,779,746 | 94,779,765 | TGGCGGATTCAGGCTTTGTC |
PCR Primers
| Primer | Sequence |
| SU | TAACCCTGCTAACCCACCAG |
| SD | ACATAGGGCAGATAGCACCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17113A-E4 | VGB6 | C57BL/6NTac | |
| 17113C-F3 | VGB6 | C57BL/6NTac | |
| 17113C-C9 | VGB6 | C57BL/6NTac | |
