| Gene | D17H6S56E-3 |
| Chromosome | 17 |
| Deletion Start | 35,026,048 |
| Deletion End | 35,017,075 |
| Deletion Size | 8,974 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,017,789 | 35,017,806 | GCCAATGCAGCCCAAGAC |
| TUP | 35,017,807 | 35,017,831 | TTCCTGCCAGCTTTCAAGAGCAACC |
| TUR | 35,017,840 | 35,017,860 | CAGCCGTTCAGCATCGAAGTG |
| TDF | 35,025,282 | 35,025,301 | TTCCTGCTAGGCTGCACTTC |
| TDP | 35,025,303 | 35,025,330 | TGGTAACACAGGAACAGGACATCAAGCC |
| TDR | 35,025,347 | 35,025,366 | CGTGACCATCCCACCCATAG |
PCR Primers
| Primer | Sequence |
| SU | AGCTTCCAGGTAGGAGAGGC |
| SD | CCTGCACTGGTGAGTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17114A-C10 | VGB6 | C57BL/6NTac | |
| 17114A-C11 | VGB6 | C57BL/6NTac | |
| 17114A-G3 | VGB6 | C57BL/6NTac | |
| 17114A-G10 | VGB6 | C57BL/6NTac | |
| 17114A-H12 | VGB6 | C57BL/6NTac | |
| 17114A-H8 | VGB6 | C57BL/6NTac | |
