| Gene | Ephx1 |
| Chromosome | 1 |
| Deletion Start | 181,001,978 |
| Deletion End | 180,989,768 |
| Deletion Size | 12,211 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,001,067 | 181,001,086 | TGGCCCATCCTTCCTTTCAC |
| TUP | 181,001,041 | 181,001,066 | CCTGTGATCCTTGGGCAGCTACTGTG |
| TUR | 181,001,012 | 181,001,031 | GACTTCCCAGCACTCCACAG |
| TDF | 180,990,598 | 180,990,619 | GAGGTAGGCATTGTCTGTTGTG |
| TDP | 180,990,573 | 180,990,596 | TGACCCTGAAGAGGGCAGCCCTTT |
| TDR | 180,990,537 | 180,990,555 | TGCCCAGGCTCAGTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CATGAATAGGTCCGGGAGTG |
| SD | TCTCAATGACAACTGCCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17116A-B10 | VGB6 | C57BL/6NTac | |
| 17116A-F11 | VGB6 | C57BL/6NTac | |
| 17116A-F2 | VGB6 | C57BL/6NTac | |
| 17116A-D10 | VGB6 | C57BL/6NTac | |
| 17116A-C10 | VGB6 | C57BL/6NTac | |
