| Gene | Fam159b |
| Chromosome | 13 |
| Deletion Start | 104,863,710 |
| Deletion End | 104,845,686 |
| Deletion Size | 18,025 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,863,405 | 104,863,426 | CCGGGAAAGTGACCAGTGAAAG |
| TUP | 104,863,376 | 104,863,403 | CACAAAGCTGGAATCAGGGAAGTCTAGC |
| TUR | 104,863,349 | 104,863,366 | TGGCACGAGGACCTGAAC |
| TDF | 104,846,059 | 104,846,081 | GGTCTTGTGTATTTCCAGGCAAC |
| TDP | 104,846,029 | 104,846,056 | AAACAGAAAAGCCAAAGGCCTCAATGCA |
| TDR | 104,845,980 | 104,846,002 | GAGTCCCATCGTCAGCTTCATAG |
PCR Primers
| Primer | Sequence |
| SU | CTGGAAGGGAGTCCGTAG |
| SD | CAAACCCACGTATTGCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17117A-B9 | VGB6 | C57BL/6NTac | |
| 17117A-E2 | VGB6 | C57BL/6NTac | |
| 17117A-A4 | VGB6 | C57BL/6NTac | |
