| Gene | Fgfr4 |
| Chromosome | 13 |
| Deletion Start | 55,168,259 |
| Deletion End | 55,155,493 |
| Deletion Size | 12,767 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,155,504 | 55,155,523 | GGCCCTGTTGAGCATCTTTC |
| TUP | 55,155,529 | 55,155,552 | ACACCAGCTTTGTCCCTTGAGGCC |
| TUR | 55,155,572 | 55,155,591 | TCCCACCTTAGAGGCCATAC |
| TDF | 55,168,085 | 55,168,103 | CACCTGCTCCTCCAGTGAC |
| TDP | 55,168,109 | 55,168,130 | TTTCAGCCACGACCCTTTGCCC |
| TDR | 55,168,188 | 55,168,209 | CCGCGTAGCCCATACAACATTG |
PCR Primers
| Primer | Sequence |
| SU | CACCATCTCTGCTTCCTTCC |
| SD | AGGCCTCTAGGTTGTTTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17119A-B3 | VGB6 | C57BL/6NTac | |
| 17119A-B10 | VGB6 | C57BL/6NTac | |
| 17119A-D2 | VGB6 | C57BL/6NTac | |
| 17119A-D11 | VGB6 | C57BL/6NTac | |
| 17119A-F10 | VGB6 | C57BL/6NTac | |
| 17119A-D5 | VGB6 | C57BL/6NTac | |
