| Gene | Frzb |
| Chromosome | 2 |
| Deletion Start | 80,446,837 |
| Deletion End | 80,413,271 |
| Deletion Size | 33,567 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,446,706 | 80,446,724 | CATCCCGCTGTGCAAGTCC |
| TUP | 80,446,679 | 80,446,703 | TCCCTGGAACATGACCAAGATGCCC |
| TUR | 80,446,648 | 80,446,666 | CGTTAGCCTGGGTGCTGTG |
| TDF | 80,413,387 | 80,413,407 | TCAGAAGTCTGGCAGGAACTC |
| TDP | 80,413,360 | 80,413,381 | CCCGGCCAGCACGCAGCTAAAT |
| TDR | 80,413,306 | 80,413,326 | CAGCAGCGCCAGTCTTAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTCTGGCTTCCTCTTCTGC |
| SD | ATGGCCAAAGGCATACAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17120A-G5 | VGB6 | C57BL/6NTac | |
| 17120A-A10 | VGB6 | C57BL/6NTac | |
| 17120A-B3 | VGB6 | C57BL/6NTac | |
| 17120A-G12 | VGB6 | C57BL/6NTac | |
| 17120A-H8 | VGB6 | C57BL/6NTac | |
| 17120A-E10 | VGB6 | C57BL/6NTac | |
