| Gene | Galr2 |
| Chromosome | 11 |
| Deletion Start | 116,283,670 |
| Deletion End | 116,281,489 |
| Deletion Size | 2,182 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,281,742 | 116,281,762 | CCCTGGACGATTGGGTGTTTG |
| TUP | 116,281,764 | 116,281,787 | CTCACTGCTCTGCAAGGCCGTTCA |
| TUR | 116,281,803 | 116,281,822 | AGCTGCTGGCGTGCATAGTG |
| TDF | 116,283,234 | 116,283,253 | AGCGCAAGGTGACACGGATG |
| TDP | 116,283,256 | 116,283,280 | CGTCATCGTGGCGGTACTCTTCTGC |
| TDR | 116,283,311 | 116,283,330 | CGACCAAACCACACGCAGAG |
PCR Primers
| Primer | Sequence |
| SU | ACCACAGGGCCAGTAATCTG |
| SD | AGTCCCACACACATCACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17121A-A8 | VGB6 | C57BL/6NTac | |
| 17121A-B6 | VGB6 | C57BL/6NTac | |
| 17121A-B10 | VGB6 | C57BL/6NTac | |
| 17121A-E4 | VGB6 | C57BL/6NTac | |
| 17121A-F2 | VGB6 | C57BL/6NTac | |
| 17121A-A11 | VGB6 | C57BL/6NTac | |
