| Gene | Gpr6 |
| Chromosome | 10 |
| Deletion Start | 41,071,581 |
| Deletion End | 41,070,427 |
| Deletion Size | 1,155 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,071,125 | 41,071,143 | TTCCTGGTGGCGTCCTTTG |
| TUP | 41,071,101 | 41,071,123 | CGCCTCAGTCAGCAGCCTGCTTG |
| TUR | 41,071,043 | 41,071,062 | CGGCGCGAGTAGTAGGTGAG |
| TDF | 41,070,650 | 41,070,668 | AGGACCCGGCCATCTACAC |
| TDP | 41,070,626 | 41,070,646 | TGCCACCCTGTTGCCTGCCAC |
| TDR | 41,070,579 | 41,070,598 | CCTGGTTGCGGAAGGCATAG |
PCR Primers
| Primer | Sequence |
| SU | TGACCACTGCACCTTAGCAG |
| SD | CTGTCTTGGCATTTCAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17123A-B2 | VGB6 | C57BL/6NTac | |
| 17123A-D9 | VGB6 | C57BL/6NTac | |
| 17123A-B11 | VGB6 | C57BL/6NTac | |
| 17123A-F8 | VGB6 | C57BL/6NTac | |
