| Gene | Ifi27l2b |
| Chromosome | 12 |
| Deletion Start | 103,457,156 |
| Deletion End | 103,450,996 |
| Deletion Size | 6,161 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,456,787 | 103,456,811 | GATGGAATTGGTGAAGAATGGGAAG |
| TUP | 103,443,225 | 103,443,250 | CAGCTGCTGAACACTGCAGGGTTGTA |
| TUR | 103,456,676 | 103,456,698 | GATGTGACAGAGCTGTGGTGTTG |
| TDF | 103,451,369 | 103,451,388 | AGGTCATTGCCTGTCACTGC |
| TDP | 103,451,344 | 103,451,366 | TGCCTCCTTCCCAGGGATCCTTG |
| TDR | 103,451,308 | 103,451,331 | CAGCTCCAAGGATGATATTGGTTG |
PCR Primers
| Primer | Sequence |
| SU | TTGCGGAGTTTGCACTTATG |
| SD | ACGTCTGGTCCATGAACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 136 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17125A-A3 | VGB6 | C57BL/6NTac | |
| 17125A-A10 | VGB6 | C57BL/6NTac | |
| 17125A-D6 | VGB6 | C57BL/6NTac | |
| 17125A-B1 | VGB6 | C57BL/6NTac | |
| 17125A-F6 | VGB6 | C57BL/6NTac | |
